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Journal / Biology / Generazione di monostrati epiteliali del colon…
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Generazione di monostrati epiteliali del colon primario murino da cripte intestinali
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Generazione di monostrati epiteliali del colon primario murino da cripte intestinali

Article DOI: 10.3791/62156-v • 06:57 min • February 6th, 2021
February 6th, 2021 •
1, 1, 1, 1, 1
1Department of Pathology, School of Medicine, University of Michigan

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In questo protocollo, descriviamo come generare monostrati epiteliali primari murini direttamente dalle cripte intestinali. Forniamo approcci sperimentali per generare monostrati confluenti su filtri permeabili, monostrati confluenti per la guarigione delle ferite da graffio e studi biochimici e monostrati sparsi e confluenti per l'analisi dell'immunofluorescenza.

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Biologia Numero 168 cultura primaria enteroidi 2D colonidi 2D cellule epiteliali monostrati epiteliali cripte intestinali
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