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Journal / Neuroscience / ROS Live Cell Imaging Under Neuronal Development
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JoVE Journal Neuroscience
ROS Live Cell Imaging Under Neuronal Development
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ROS Live Cell Imaging Under Neuronal Development

Article DOI: 10.3791/62165-v • 09:25 min • February 9th, 2021
February 9th, 2021 •
1,2, 1,2, 1,2,3
1Department of Biological Sciences, Purdue University, 2Purdue Institute for Integrative Neuroscience, Purdue University, 3Bindley Bioscience Center, Purdue University

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Denne protokol beskriver brugen af en genetisk kodet brintoverilte (H2O2)-biosensor i dyrkede zebrafisk neuroner og larver til vurdering af de fysiologiske signalroller af H2O2 under udvikling af nervesystemet. Det kan anvendes til forskellige celletyper og modificeres med eksperimentelle behandlinger for at studere reaktive iltarter (ROS) i generel udvikling.

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Neurovidenskab ROS brintoverilte roGFP2-Orp1 biosensor RGC zebrafisk neuronal udvikling
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