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En 3D-kartografisk beskrivning av cellen genom Cryo Soft X-ray Tomography
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En 3D-kartografisk beskrivning av cellen genom Cryo Soft X-ray Tomography

Article DOI: 10.3791/62190-v • 08:47 min • March 15th, 2021
March 15th, 2021 •
1, 1, 1, 1, 1, 2, 2, 2, 1, 1
1MISTRAL beamline, Alba Light Source, Cerdanyola del Valles, Spain, 2Beamline B24, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

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Här presenteras ett protokoll som beskriver provberedningen och datainsamlingsstegen som krävs i kryo mjuk röntgentomografi (SXT) för att avbilda ultrastrukturen hos hela kryobevarade celler med en upplösning av 25 nm halv tonhöjd.

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Biologi utgåva 169 röntgenavbildning mjuk röntgentomografi kryogena temperaturer hela cellen spektromikroskopi
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