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Journal / Biology / TGF-β mediert endotelial til mesenchymal overgang …
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Biology

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TGF-β mediert endotelial til mesenchymal overgang (EndMT) og den funksjonelle vurderingen av EndMT-effektorer ved hjelp av CRISPR/Cas9 Genredigering
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TGF-β mediert endotelial til mesenchymal overgang (EndMT) og den funksjonelle vurderingen av EndMT-effektorer ved hjelp av CRISPR/Cas9 Genredigering

Article DOI: 10.3791/62198-v • 07:05 min • February 26th, 2021
February 26th, 2021 •
1,2, 1,2, 1, 1,2
1Dept. Cell Chemical Biology, Leiden University Medical Center, 2Oncode Institute, Leiden University Medical Center

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Vi beskriver metoder for å undersøke TGF-β2-indusert EndMT i endotelceller ved å observere cellemorfologiendringer og undersøke uttrykket EndMT-relaterte markørendringer ved hjelp av immunfluorescensfarging. CRISPR/Cas9 genredigering ble beskrevet og brukt til å tømme genkodingen Snail for å undersøke sin rolle i TGF-β2-indusert EndMT.

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Biologi Utgave 168 CRISPR/Cas9 knock-out lentivirus Immunfluorescence MS-1 Snegl Slug
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