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Journal / Biology / TGF-β medieret endotel til mesenchymal overgang…
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Biology

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TGF-β medieret endotel til mesenchymal overgang (EndMT) og funktionel vurdering af EndMT-effektorer ved hjælp af CRISPR/Cas9-genredigering
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TGF-β medieret endotel til mesenchymal overgang (EndMT) og funktionel vurdering af EndMT-effektorer ved hjælp af CRISPR/Cas9-genredigering

Article DOI: 10.3791/62198-v • 07:05 min • February 26th, 2021
February 26th, 2021 •
1,2, 1,2, 1, 1,2
1Dept. Cell Chemical Biology, Leiden University Medical Center, 2Oncode Institute, Leiden University Medical Center

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Vi beskriver metoder til at undersøge TGF-β2-induceret EndMT i endotelceller ved at observere cellemorfologiændringer og undersøge udtrykket EndMT-relaterede markørændringer ved hjælp af immunfluorescensfarvning. CRISPR/Cas9 genredigering blev beskrevet og brugt til at nedbryde genet kodning Snail at undersøge sin rolle i TGF-β2-induceret EndMT.

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Biologi Problem 168 CRISPR/Cas9 knock-out lentivirus Immunofluorescens MS-1 Snail Slug
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