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TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing
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TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

Article DOI: 10.3791/62198-v • 07:05 min • February 26th, 2021
February 26th, 2021 •
1,2, 1,2, 1, 1,2
1Dept. Cell Chemical Biology, Leiden University Medical Center, 2Oncode Institute, Leiden University Medical Center

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Summary
Automatic Translation

We describe methods to investigate TGF-β2-induced EndMT in endothelial cells by observing cell morphology changes and examining the expression EndMT-related marker changes using immunofluorescence staining. CRISPR/Cas9 gene editing was described and used to deplete the gene encoding Snail to investigate its role in TGF-β2-induced EndMT.

Tags

TGF-beta Endothelial To Mesenchymal Transition EndMT Effectors CRISPR/Cas9 Gene Editing Cell Morphology Endothelial Markers Mesenchymal Markers Diseases Therapeutic Benefit Embryogenesis Organ Development Intracellular Protein Immunofluorescence Staining Cell Permeabilization Antibodies Cytokines
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