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Journal / Immunology and Infection / 인간 CRISPR-엔지니어링 CAR-T 셀 생산
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
인간 CRISPR-엔지니어링 CAR-T 셀 생산
Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
Journal (Immunology and Infection)
Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
piggyBac Transposon System Modification of Primary Human T Cells
Journal (Immunology and Infection)
piggyBac Transposon System Modification of Primary Human T Cells
 
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인간 CRISPR-엔지니어링 CAR-T 셀 생산

Article DOI: 10.3791/62299-v • 06:33 min • March 15th, 2021
March 15th, 2021 •
*1,2, *1,2, 1,2, 1,2
1Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, 2Parker Institute for Cancer Immunotherapy, University of Pennsylvania
* These authors contributed equally

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여기서, 우리는 CAR-T 세포를 수정하기 위하여 CRISPR Cas 기술을 사용하여 1 차적인 인간 T 세포에 있는 유전자 편집을 위한 프로토콜을 제시합니다.

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면역학 및 감염 문제 169 키메라 항원 수용체 (CAR)-T 세포 세포 면역 요법 유전자 치료 렌즈 바이러스 벡터 유전자 편집 CRISPR /Cas9 T 림프구
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