Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Practical Aspects of Sample Preparation and Setup …
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
Journal (Biochemistry)
Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
Locus-Specific Chromatin Purification from Saccharomyces cerevisiae (Video) | JoVE
Journal (Biochemistry)
Locus-Specific Chromatin Purification from Saccharomyces cerevisiae (Video) | JoVE
 

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Article DOI: 10.3791/62470-v • 08:17 min • July 9th, 2021
July 9th, 2021 •
1, 1, 1
1Department of Medical Biochemistry and Biophysics, Karolinska Institutet

Chapters

Summary
Automatic Translation

We present a protocol to measure micro- to millisecond dynamics on 13C/15N-labeled and unlabeled RNA with 1H R1ρ relaxation dispersion nuclear magnetic resonance (NMR) spectroscopy. The focus of this protocol lies in high-purity sample preparation and setup of NMR experiments.

Tags

RNA Sample Preparation NMR Setup Proton R1rho Relaxation Dispersion Confirmational Exchange Shifted Base-pairing Isotopic Labeling In Vitro Transcription Cleavage Reaction Denaturing PAGE Gel Incomplete Cleavage Microwave Heating Reanneal RNA Inorganic Pyrophosphatase RNase H
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter