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Journal / Biochemistry / Produktion af rekombinante PRMT-proteiner ved…
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Biochemistry
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JoVE Journal Biochemistry
Produktion af rekombinante PRMT-proteiner ved hjælp af Baculovirus Expression Vector System
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Produktion af rekombinante PRMT-proteiner ved hjælp af Baculovirus Expression Vector System

Article DOI: 10.3791/62510-v • 08:57 min • July 17th, 2021
July 17th, 2021 •
1, 1
1Structural Genomics Consortium, University of Toronto

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Baculovirusudtryksvektorsystemet (BEVS) er en robust platform for ekspressionsscreening og produktion af proteinalgininmethyletransferaser (PRMT'er), der skal anvendes til biokemiske, biofysiske og strukturelle undersøgelser. Milligram mængder materiale kan produceres for de fleste PRMT'er og andre proteiner af interesse, der kræver en eukaryote udtryksplatform.

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Biokemi Udsendelse 173 proteinalgininmethyletransferaser PRMT'er baculovirusudtrykvektorsystem BEVS Spodoptera frugiperda insektceller rekombinant protein transfektreagenser suspensionskultur af baculovirusinficerede insektceller SCBIIC 2.8 L Fernbach KulturKolbe 2,5 L Tunair-kolbe 5 L reagensflasker
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