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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
This content is Free Access.
Chapters
- 00:00Introduciton
- 00:28Generation of a Recombinant Baculovirus Stocks
- 03:12Small-scale Proteins Expression Screening and Virus Amplificaiton
- 05:26Infection of the Sf9 with SCBIIC for Protein Production
- 06:09Results
- 06:51Conclusion
The baculovirus expression vector system (BEVS) is a robust platform for expression screening and production of protein arginine methyltransferases (PRMTs) to be used for biochemical, biophysical, and structural studies. Milligram quantities of material can be produced for the majority of PRMTs and other proteins of interest requiring a eukaryotic expression platform.
