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JoVE Journal
Developmental Biology

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Nuklear migration i Drosophila Oocyte
 
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Nuklear migration i Drosophila Oocyte

Article DOI: 10.3791/62688-v 04:17 min May 13th, 2021
May 13th, 2021

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Summary

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Click here for the English version.

I Drosophilagennemgår oocytkernen mikrotubuleafhængig migration under oogenesis. Her beskriver vi en protokol, der blev udviklet til at følge migrationen ved at udføre live billeddannelse på ægkamre ex-vivo. Vores procedure opretholder ægkamre i live i 12 timer for at erhverve multi-position 3D time-lapse film ved hjælp af spinning-disk confocal mikroskopi.

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Udviklingsbiologi Problem 171 nuklear migration kernepositionering Drosophila oocyt mikrotubuli levende billeddannelse
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