Journal
/
Developmental Biology
/
Celle sammenlægning Assays til at evaluere bindingen af Drosophila Notch med Trans-ligander og dens hæmning af Cis-ligander
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Celle sammenlægning Assays til at evaluere bindingen af Drosophila Notch med Trans-ligander og dens hæmning af Cis-ligander

English

Automatically Generated

7,404 Views

•

05:48 min

•

January 02, 2018

DOI:

10.3791/56919-v

DOI:
10.3791/56919-v

•

05:48 min
January 02, 2018

•

5 Views
,
1Department of Molecular and Human Genetics,Baylor College of Medicine, 2Program in Developmental Biology,Baylor College of Medicine

Summary

Automatically generated

I vivo systemers kompleksitet gør det vanskeligt at skelne mellem aktivering og hæmning af Notch receptor af trans- og cis-ligander, henholdsvis. Vi præsenterer her, en protokol baseret på in vitro- celle-sammenlægning assays til kvalitative og semi-kvantitative evaluering af bindingen af Drosophila hak til trans-ligander vs cis-ligander.

Explore More Videos

Cell Aggregation AssayNotch ReceptorTrans-ligandsCis-ligandsDrosophilaS2 CellsLigand BindingCell SignalingAggregation QuantificationMicroscopyHemacytometer

Read Article

Cite this Article

Pandey, A., Jafar-Nejad, H. Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands. J. Vis. Exp. (131), e56919, doi:10.3791/56919 (2018).

Download .ris file

Copy

Share Video

.

Copy