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Propagation of Dental and Respiratory Cells and Organs in Microgravity
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Bioengineering
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JoVE Journal Bioengineering
Propagation of Dental and Respiratory Cells and Organs in Microgravity

Propagation of Dental and Respiratory Cells and Organs in Microgravity

DOI:
10.3791/62690-v

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06:29 min

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May 25, 2021

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, , , ,
1Center for Craniofacial Research and Diagnosis,Texas A&M College of Dentistry, 2Department of Stomatology,The Fourth Affiliated Hospital, Harbin Medical University, 3Key Laboratory of Oral Medicine,Guangzhou Insititute of Oral Disease, Stomatology Hospital of Guangzhou Medical University

Chapters

  • 00:04Introduction
  • 00:55Cervical Loop (CL) Dissection, Co-Culturing, and Periodontal Progenitor Cells on a Scaffold Within the Rotary Bioreactor Systems (RCCS)
  • 02:53Results: Analysis of Cervical Loop Cells Co-Cultured with Dental Pulp Stem Cells in a Bioreactor
  • 05:02Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a method for the culture and 3D growth of ameloblast-like cells in microgravity to maintain their elongated and polarized shape as well as enamel-specific protein expression. Culture conditions for the culture of periodontal engineering constructs and lung organs in microgravity are also described.

Tags

Keywords: MicrogravityDental CellsRespiratory CellsAmeloblastEnamelBioreactorScaffoldMedia ChangeMouse MandibleCervical LoopDental PulpEnamel Research

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