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Propagation of Dental and Respiratory Cells and Organs in Microgravity

Article DOI: 10.3791/62690-v • 06:29 min • May 25th, 2021
May 25th, 2021 •
1, 1,2, 1,3, 1, 1
1Center for Craniofacial Research and Diagnosis, Texas A&M College of Dentistry, 2Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, 3Key Laboratory of Oral Medicine, Guangzhou Insititute of Oral Disease, Stomatology Hospital of Guangzhou Medical University

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Summary
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This protocol presents a method for the culture and 3D growth of ameloblast-like cells in microgravity to maintain their elongated and polarized shape as well as enamel-specific protein expression. Culture conditions for the culture of periodontal engineering constructs and lung organs in microgravity are also described.

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Propagation Dental Cells Respiratory Cells Organs Microgravity Ameloblasts Enamel Field Bioreactor Model Enamel Research Tissue Collection Cervical Loops Dental Pulp Scaffold Keratinocyte SFM Media Growth Factors Extracellular Matrix Proteins Syringe Valves Media Change
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