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Bioengineering

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JoVE Journal Bioengineering
Formering af tand- og åndedrætsceller og organer i mikrogravity
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Formering af tand- og åndedrætsceller og organer i mikrogravity

Article DOI: 10.3791/62690-v • 06:29 min • May 25th, 2021
May 25th, 2021 •
1, 1,2, 1,3, 1, 1
1Center for Craniofacial Research and Diagnosis, Texas A&M College of Dentistry, 2Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, 3Key Laboratory of Oral Medicine, Guangzhou Insititute of Oral Disease, Stomatology Hospital of Guangzhou Medical University

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Denne protokol præsenterer en metode til dyrkning og 3D-vækst af ameloblast-lignende celler i mikrogravity for at opretholde deres aflange og polariserede form samt emaljespecifikke proteinekspression. Kulturbetingelser for kulturen af periodontale ingeniørkonstruktioner og lungeorganer i mikrogravitet er også beskrevet.

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