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Producción rápida, asequible y sin complicaciones de lisado bacteriano libre de células

Article DOI: 10.3791/62753-v • 06:03 min • October 29th, 2021
October 29th, 2021 •
*1, *1,2, *1,3, 1,4,5
1Biocircuits Institute, University of California, San Diego, 2Present address, Kyoto Institute of Technology, 3Present address, Vertex Pharmaceuticals, 4Department of Bioengineering, University of California, San Diego, 5Molecular Biology Section, University of California, San Diego
* These authors contributed equally

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Este protocolo describe un método rápido y simple para producir lisado bacteriano para la expresión génica libre de células, utilizando una cepa modificada de Escherichia coli y requiriendo solo equipo de laboratorio estándar.

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Biología Número 176
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