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Isolation and Characterization of the Natural Microbiota of the Model Nematode Caenorhabditis elegans
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Characterization of the Natural Microbiota of the Model Nematode Caenorhabditis elegans
DOI:

07:05 min

August 17, 2022

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Chapters

  • 00:05Introduction
  • 00:45Preparation of Environmental Samples
  • 01:10Isolation of Caenorhabditis Nematodes
  • 02:07Preparation of the Worms for Molecular Identification of Caenorhabditis elegans and Microbes
  • 03:18Isolation and Cultivation of Nematode-Associated Bacteria
  • 05:04Results: Characterization of the Caenorhabditis elegans Microbiota
  • 06:15Conclusion

Summary

Automatic Translation

Caenorhabditis elegans is one of the main model species in biology, yet almost all research is performed in the absence of its naturally associated microbes. The methods described here will help to improve our understanding of the diversity of associated microbes as a basis for future functional C. elegans research.

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