Journal
/
Biology
/
Kvantificering af interbakteriel konkurrence ved hjælp af enkeltcellet fluorescens imaging
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging

Kvantificering af interbakteriel konkurrence ved hjælp af enkeltcellet fluorescens imaging

DOI:
10.3791/62851-v

•

07:34 min

•

September 02, 2021

•

,
1Department of Earth, Marine, and Environmental Sciences,University of North Carolina

Chapters

  • 00:05Introduction
  • 00:39Agarose Pad Preparation
  • 01:47Co-Incubation Strain Preparation
  • 02:37Bacterial Strains Coincubation
  • 03:35Slide Setup
  • 04:17Fluorescence Microscopy
  • 04:46Image Analysis
  • 05:50Results: Fluorescence Image Analysis to Assess Target Strain Inhibition in Coincubation Assay
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskript beskriver en metode til brug af enkeltcellet fluorescensmikroskopi til at visualisere og kvantificere bakteriekonkurrence i coculture.

Tags

Keywords: QuantificationInterbacterial CompetitionSingle-cell Fluorescence ImagingType VI Secretion SystemBacterial CompetitionCell AreaCulturable MicrobesAgarose PadLBS AgarOptical DensityNormalizationStrain Mixing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62851?article_type=v

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/62851?article_type=v

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
check_url/62851?article_type=v

Erhvervelse Fluorescens Time-lapse film fra Spirende Gær og analysere encellede Dynamics hjælp poder
check_url/62851?article_type=v

Sammenligning affiniteten af ​​GTPase-bindende proteiner under anvendelse af kompetitive assays
check_url/62851?article_type=v

CUBIC protokol Visualiserer Protein Expression på Single Cell Opløsning i Whole Mount Skin Forberedelser
check_url/62851?article_type=v

Funktionel billeddannelse af Viral transskription fabrikker ved hjælp af 3D Fluorescens mikroskopi
check_url/62851?article_type=v

Analyse af Beta-celle funktion ved hjælp af encellede opløsning Calcium Imaging i zebrafisk Holme
check_url/62851?article_type=v

Encellet opløsning tredimensionel billeddannelse af intakte organoider
check_url/62851?article_type=v

Rekonstruktion af enkeltcellede medfødte fluorescenssignaturer af konfokal mikroskopi
check_url/62851?article_type=v

Enkeltcellet multiplexed fluorescensbilleddannelse for at visualisere virale nukleinsyrer og proteiner og overvåge HIV, HTLV, HBV, HCV, Zika-virus og influenzainfektion

Read Article