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Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets
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Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

DOI:
10.3791/57851-v

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08:50 min

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July 03, 2018

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, ,
1Center for Molecular and Cellular Bioengineering,TU Dresden, 2Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus of TU Dresden

Chapters

  • 00:04Title
  • 00:33Zebrafish Primary Islet Dissection and Mounting
  • 02:20Ex Vivo Live-imaging of GCaMP Fluorescence Intensity in Zebrafish Primary Islets
  • 05:11Quantification of GCaMP Fluorescence Trace for Individual Beta-cells
  • 07:14Results: Single-cell Calcium Imaging in Zebrafish Islet Beta Cells
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

Beta-cell functionality is important for blood-glucose homeostasis, which is evaluated at single-cell resolution using a genetically encoded reporter for calcium influx.

Tags

Keywords: Beta-cellCalcium ImagingZebrafish IsletsBeta-cell FunctionCalcium OscillationsSingle-cell ResolutionEx Vivo Live ImagingGCaMP FluorescenceFibrinogen-thrombin MoldMKO2 Fluorescence

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