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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow cytometrisk analyse for identifisering av de medfødte og adaptive immuncellene i Murine Lung
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Flow cytometrisk analyse for identifisering av de medfødte og adaptive immuncellene i Murine Lung

Article DOI: 10.3791/62985-v • 09:57 min • November 16th, 2021
November 16th, 2021 •
1,2, 1,2,3, 1,2, 1,2, 1,2
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

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I denne studien presenterer vi en effektiv og reproduserbar protokoll for å isolere immunpopulasjonene i murin respiratorisk system. Vi tilbyr også en metode for identifisering av alle medfødte og adaptive immunceller som ligger i lungene til friske mus, ved hjelp av et 9-fargebasert strømningscytometripanel.

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Immunologi og infeksjon utgave 177 lunge immunceller gatingstrategi strømningscytometri
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