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1LumiSTAR Biotechnology, Inc., 2NEXEL Co., Ltd., 3Institute of Pharmacology, College of Medicine,National Taiwan University, 4Manchester Heart Centre, Manchester Royal Infirmary,Manchester University NHS Foundation Trust, 5Division of Cardiovascular Sciences, Manchester Academic Health Sciences Centre,University of Manchester, 6Division of Cell Matrix Biology and Regenerative Medicine,University of Manchester
Chapters
- 00:04Introduction
- 00:57iPSC Derived Cardiomyocytes Preparation
- 02:28Expression of Genetically Encoded Calcium Indicators
- 03:24Image Acquisition and Functional Assays for Toxicity Test and Phenotypic Screening
- 05:22Small Molecule Preparation
- 05:58Imaging Processing and Analysis
- 06:31Results: Imaging and Processing of Cells
- 09:28Conclusion
The present protocol describes all-optical control and observation of triggered cellular activity in iPSC-derived cardiomyocytes (iPSC-CMs) for high throughput drug screening and toxicity testing. Multi-parametric quantification of phenotypic patterns in time, and space, are shown. Long-term effects of drugs over hours, or sequential measurements over days, are demonstrated.
