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High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening
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High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening

Article DOI: 10.3791/63175-v • 10:01 min • March 31st, 2022
March 31st, 2022 •
1, 1, 1, 1, 2, 3, 3, 3, 4,5,6
1LumiSTAR Biotechnology, Inc., 2NEXEL Co., Ltd., 3Institute of Pharmacology, College of Medicine, National Taiwan University, 4Manchester Heart Centre, Manchester Royal Infirmary, Manchester University NHS Foundation Trust, 5Division of Cardiovascular Sciences, Manchester Academic Health Sciences Centre, University of Manchester, 6Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester

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Summary
Automatic Translation

The present protocol describes all-optical control and observation of triggered cellular activity in iPSC-derived cardiomyocytes (iPSC-CMs) for high throughput drug screening and toxicity testing. Multi-parametric quantification of phenotypic patterns in time, and space, are shown. Long-term effects of drugs over hours, or sequential measurements over days, are demonstrated.

Tags

Optical Control Calcium Signal IPSC-derived Cardiomyocytes Toxicity Testing Phenotypic Drug Screening High-throughput Multi-parametric Quantification Functional Screening Gelatin Solution Fibronectin Liquid Nitrogen Storage ROCK Inhibitor Y27632 96 Well Plates
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