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Journal / Biology / 用超极化[1-13C]丙酮酸和 13C / 31P…
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用超极化[1-13C]丙酮酸和 13C / 31P NMR波谱研究分离灌注小鼠心脏中的心脏代谢
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用超极化[1-13C]丙酮酸和 13C / 31P NMR波谱研究分离灌注小鼠心脏中的心脏代谢

Article DOI: 10.3791/63188-v • 14:56 min • April 21st, 2023
April 21st, 2023 •
1,2, 1,2, 1, 1, 1, 1,2
1Department of Radiology, Hadassah Medical Organization and Faculty of Medicine, Hebrew University of Jerusalem, 2The Wohl Institute for Translational Medicine

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我们描述了一种实验装置，用于在连续灌注模式下向分离的灌注小鼠心脏施用超极化 13种C标记代谢物。专用的 13C-NMR采集方法能够实时定量代谢酶活性，多参数 31P-NMR分析可以测定组织ATP含量和pH值。

Tags

生物学，第194期，代谢，代谢成像，成像剂，磁共振波谱，乳酸脱氢酶，丙酮酸脱氢酶，[1-13C]丙酮酸，pH，无机磷酸盐
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