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Journal / Biology / Undersøgelse af hjertemetabolisme i det isolerede …
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Undersøgelse af hjertemetabolisme i det isolerede perfunderede musehjerte med hyperpolariseret [1-13 C] pyruvat og 13C / 31P NMR spektroskopi
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Undersøgelse af hjertemetabolisme i det isolerede perfunderede musehjerte med hyperpolariseret [1-13 C] pyruvat og 13C / 31P NMR spektroskopi

Article DOI: 10.3791/63188-v • 14:56 min • April 21st, 2023
April 21st, 2023 •
1,2, 1,2, 1, 1, 1, 1,2
1Department of Radiology, Hadassah Medical Organization and Faculty of Medicine, Hebrew University of Jerusalem, 2The Wohl Institute for Translational Medicine

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Vi beskriver en eksperimentel opsætning til administration af hyperpolariserede 13C-mærkede metabolitter i kontinuerlig perfusionstilstand til et isoleret perfuseret musehjerte. En dedikeret 13C-NMR-erhvervelsesmetode muliggjorde kvantificering af metabolisk enzymaktivitet i realtid, og en multiparametrisk 31P-NMR-analyse muliggjorde bestemmelse af vævets ATP-indhold og pH.

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Biologi udgave 194 Metabolisme metabolisk billeddannelse billeddannelsesmiddel magnetisk resonansspektroskopi lactat dehydrogenase pyruvat dehydrogenase [1-13C] pyruvat pH uorganisk phosphat
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