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JoVE Journal
Biochemistry

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福斯特共振能量转移映射:阐明全球结构特征的新方法
 
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福斯特共振能量转移映射:阐明全球结构特征的新方法

Article DOI: 10.3791/63433-v 07:09 min March 16th, 2022
March 16th, 2022

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Summary

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Click here for the English version.

该研究详细介绍了FRET映射的方法,包括标记位点的选择,染料的选择,采集和数据分析。该方法可有效确定蛋白质系统中的结合位点、构象变化和动态运动,如果与现有的3-D结构信息结合使用,这种方法最有用。

Tags

生物化学,第181期,荧光,能量转移,SecA,蛋白质易位,FRET映射
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