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Biochemistry
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定量荧光测量全球本地化翻译
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Quantitative Immunofluorescence to Measure Global Localized Translation
Please note that all translations are automatically generated.
Click here for the English version.
定量荧光测量全球本地化翻译
DOI:
10.3791/55909-v
•
09:13 min
•
August 22, 2017
•
Jonathan Bergeman
,
Marc-Étienne Huot
2
1
Centre de Recherche sur le Cancer de l’Université Laval, Faculté de Médecine, Département de Biologie moléculaire, biochimie médicale et pathologie
,
Université Laval
,
2
CRCHU de Québec: L’Hôtel-Dieu de Québec
Chapters
00:05
Title
00:45
Cell Immunostaining
03:34
Image Acquisition
04:49
Signal Quantification
06:50
Results: Proper Puromycin Treatment and Analysis in MRC-5 and Other Cell Types
08:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这份手稿描述了一种方法来可视化和量化本地化的翻译事件亚细胞的车厢内。提出这份手稿的做法需要基本的共焦成像系统和试剂,并且是快速和具有成本效益。
Tags
Quantitative Immunofluorescence
Localized Translation
Cell Adhesion
Puromycin
Anti-puromycin Antibody
Fluorophore-conjugated Secondary Antibodies
Cell Migration
Cell Adhesion
Early Drug Response
MRC-5 Cells
Cycloheximide
Formaldehyde Fixation
Triton X-100 Permeabilization
BSA Blocking
Tween-20 Washing
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