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Kwantitatieve analyse van visco-elastische eigenschappen van rode bloedcellen met behulp van een optisch pincet en onscherpe microscopie
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Kwantitatieve analyse van visco-elastische eigenschappen van rode bloedcellen met behulp van een optisch pincet en onscherpe microscopie

Article DOI: 10.3791/63626-v • 08:03 min • March 25th, 2022
March 25th, 2022 •
*1,2, *1,2, 1,2, 1,2, 1,3, 4,5, 6, 1,2, 1,2,3,7
1Centro Nacional de Biologia Estrutural e Bioimagem - CENABIO, Universidade Federal do Rio de Janeiro, 2Instituto de Física, Programa de Pós-graduação Multidisciplinar em Física Aplicada, Universidade Federal do Rio de Janeiro, 3Instituto de Biofísica Carlos Chagas Filho, Programa de Pós-graduação em Ciências Biológicas Biofísica, Universidade Federal do Rio de Janeiro, 4Faculdade de Farmácia, Universidade Federal do Rio de Janeiro - Campus Macaé, 5Faculdade de Medicina, Programa de Pós-Graduação em Endocrinologia, Universidade Federal do Rio de Janeiro, 6Instituto de Ciências Exatas, Departamento de Física, Universidade Federal de Minas Gerais, 7Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro
* These authors contributed equally

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Hier wordt een geïntegreerd protocol op basis van een optisch pincet en defocusmicroscopie beschreven om de reologische eigenschappen van cellen te meten. Dit protocol is breed toepasbaar bij het bestuderen van de visco-elastische eigenschappen van erytrocyten onder variabele fysio-pathologische omstandigheden.

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Retractie optisch pincet defocusmicroscopie erytrocyt celvisco-elasticiteit opslagschuifmodulus verliesschuifmodulus zachte glasachtige reologie power-law exponent
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