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JoVE Journal Biochemistry
High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
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High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Article DOI: 10.3791/63664-v • 08:48 min • April 28th, 2022
April 28th, 2022 •
1,2, 2, 1,2, 3, 3, 4, 2, 1
1Institut für Angewandte Physik, Universität Tübingen, 2Institut Max von Laue - Paul Langevin (ILL), 3Institut de Biologie Structurale, Université Grenoble Alpes, 4Institut Européen de Chimie et Biologie, Bordeaux INP, Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux

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Summary
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Neutron backscattering spectroscopy offers a nondestructive and label-free access to the ps-ns dynamics of proteins and their hydration water. The workflow is presented with two studies on amyloid proteins: on the time-resolved dynamics of lysozyme during aggregation and on the hydration water dynamics of tau upon fiber formation.

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High-Resolution Neutron Spectroscopy Picosecond-Nanosecond Dynamics Proteins Hydration Water Neutron Backscatterings Radiation Damage Spatial Correlations Time Correlations Molecular Level Soft Matter Biological Materials Local Order Long Range Disorder Systems Dynamics In Biology Dynamics In Chemistry Dynamics In Materials Energy Field Batteries Fuel Cells Molecular Dynamics Of Proteins Neurodegenerative Diseases Alzheimer's Disease Parkinson's Disease Molecular Hallmarks Better Diagnostics Sample Holder Preparation
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