Journal
/
Biochemistry
/
Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

DOI:
10.3791/63856-v

•

08:31 min

•

July 20, 2022

•

, , , , , , , , , , , ,
1Department of Biomedical Engineering,Pennsylvania State University, 2Department of Materials Science and Engineering,McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, 5Materials Research Institute,Pennsylvania State University, 6Huck Institutes of the Life Sciences,Pennsylvania State University, 7Applications team,Direct Electron, 8Application Scientist,Protochips, Inc., 9Department of Biology,Wake Forest University

Chapters

  • 00:05Introduction
  • 01:43Loading the Specimen Holder for Liquid-EM
  • 02:47Production of Microchip Sandwich Assemblies
  • 03:51Imaging Specimens Using a Transmission Electron Microscope
  • 05:15Analysis of SARS-CoV-2 Sub-Viral Assemblies in Liquid
  • 06:09Results: Representative structures of AAV, SARS-CoV-2 Sub-Viral Assemblies and Rotavirus DLPs Prepared in Liquid and Vitreous Ice Using the Microchip Sandwich Technique
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

Here protocols are described to prepare virus assemblies suitable for liquid-EM and cryo-EM analysis at the nanoscale using transmission electron microscopy.

Tags

Keywords: Liquid Electron MicroscopySARS-CoV-2ProteinsVitreous IceLiquid EnvironmentsVaccine CandidatesAntibody-based TherapiesMolecular DynamicsCryo-EMLiquid EM WorkflowsMicrochip Sandwich MethodSilicon Nitride MicrochipsGlow DischargeTurbo Pumped Dry Pumping Station

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63856?article_type=v

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
check_url/63856?article_type=v

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
check_url/63856?article_type=v

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
check_url/63856?article_type=v

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
check_url/63856?article_type=v

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
check_url/63856?article_type=v

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
check_url/63856?article_type=v

Förster Resonance Energy Transfer Measurements in Living Plant Cells
check_url/63856?article_type=v

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
check_url/63856?article_type=v

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Read Article