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Vurdering af mitokondriefunktion i iskiasnerven ved respirometri i høj opløsning
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Vurdering af mitokondriefunktion i iskiasnerven ved respirometri i høj opløsning

Article DOI: 10.3791/63690-v • 08:19 min • May 5th, 2022
May 5th, 2022 •
1, 1
1Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro

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Respirometri med høj opløsning koblet til fluorescenssensorer bestemmer mitokondrie iltforbrug og reaktive iltarter (ROS) generation. Den nuværende protokol beskriver en teknik til at vurdere mitokondrie respiratoriske satser og ROS produktion i den permeabiliserede iskiasnerve.

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Biokemi udgave 183
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