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Biochemistry
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通过高分辨率呼吸测量法评估坐骨神经的线粒体功能
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Biochemistry
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Biochemistry
Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
Please note that all translations are automatically generated.
Click here for the English version.
通过高分辨率呼吸测量法评估坐骨神经的线粒体功能
DOI:
10.3791/63690-v
•
08:19 min
•
May 05, 2022
•
Marcos A. Formiga-Jr
,
Juliana Camacho-Pereira
1
Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde
,
Universidade Federal do Rio de Janeiro
Chapters
00:04
Introduction
01:19
Calibration of Polarographic Oxygen Sensors for High-Resolution Respirometry (HRR)
01:56
Dissection and Permeabilization of the Sciatic Nerve
02:58
Oxygen Consumption and Reactive Oxygen Species (ROS) Production Determination
05:51
Results: Assessing the Mitochondrial Oxygen Consumption by the Permeabilized Sciatic Nerve
07:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
高分辨率呼吸测量与荧光传感器相结合,可测定线粒体耗氧量和活性氧 (ROS) 生成。本方案描述了一种评估透化坐骨神经中线粒体呼吸频率和ROS产生的技术。
Tags
Mitochondrial Function
Sciatic Nerve
High-resolution Respirometry
Oxygen Consumption
ROS Production
Neuropathies
Diabetes
Chemotherapy
Neurobiology
Biochemistry
Pharmacology
Mitochondrial Disease Mechanisms
Mitochondrial Boosters
Neuropathic Pain
Permeabilization
Amplex Red
Peroxidase
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