Methodology to Test Control Agents and Insecticides Against the Coffee Berry Borer Hypothenemus hampei

This method uses green coffee fruits to test the toxicity of insecticides against the coffee berry borer. The insecticide application is performed before or after the insect infestation to study the insect mortality repellency and other effects. Using real green coffee with nutrients adequate for insect growth is the most appropriate way to evaluate the toxicity of compounds to insects under simulated natural conditions.

The protocol allows the evaluation of the toxic effects of chemical insecticides and toward pathogens such as fungi or repellent substances. This infection of the fruits and the insects is the critical step as improper disinfection can cause insect death due to contamination. Insect quality is also important as weak insects will overestimate the results of a toxic product.