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Biochemistry

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JoVE Journal Biochemistry
Samtidig interferensrefleksion og total intern refleksion fluorescensmikroskopi til billeddannelse af dynamiske mikrotubuli og tilhørende proteiner
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Samtidig interferensrefleksion og total intern refleksion fluorescensmikroskopi til billeddannelse af dynamiske mikrotubuli og tilhørende proteiner

Article DOI: 10.3791/63730-v • 06:43 min • May 3rd, 2022
May 3rd, 2022 •
1, 1, 1,2
1Department of Molecular Biophysics & Biochemistry, Yale University, 2Department of Physics, Yale University

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Vi præsenterer en protokol til implementering af interferens-refleksionsmikroskopi og total-intern-refleksion-fluorescensmikroskopi til samtidig billeddannelse af dynamiske mikrotubuli og fluorescerende mærkede mikrotubuli-associerede proteiner.

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Biokemi udgave 183 Etiketfri billeddannelse interferens-refleksionsmikroskopi total-intern-refleksion-fluorescensmikroskopi enkeltmolekylebilleddannelse mikrotubulidynamik kinesin
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