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Biochemistry

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JoVE Journal Biochemistry
Microscopia a fluorescenza a riflessione interna simultanea e riflessione interna totale per l'imaging di microtubuli dinamici e proteine associate
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Microscopia a fluorescenza a riflessione interna simultanea e riflessione interna totale per l'imaging di microtubuli dinamici e proteine associate

Article DOI: 10.3791/63730-v • 06:43 min • May 3rd, 2022
May 3rd, 2022 •
1, 1, 1,2
1Department of Molecular Biophysics & Biochemistry, Yale University, 2Department of Physics, Yale University

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Presentiamo un protocollo per l'implementazione della microscopia a riflessione di interferenza e della microscopia a riflessione totale interna-fluorescenza per l'imaging simultaneo di microtubuli dinamici e proteine associate a microtubuli marcati fluorescenti.

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Biochimica Numero 183 Imaging senza etichette microscopia a riflessione di interferenza microscopia a riflessione interna totale microscopia a fluorescenza a singola molecola imaging a singola molecola dinamica dei microtubuli kinesin
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