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JoVE Journal Developmental Biology
Preparación y análisis morfológico de cultivos de células de cresta neural craneal de pollo
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Preparación y análisis morfológico de cultivos de células de cresta neural craneal de pollo

Article DOI: 10.3791/63799-v • 09:26 min • June 27th, 2022
June 27th, 2022 •
1, 2,3, 2,3, 2,3
1Department of Biology and Neuroscience Program, Hamline University, 2Department of Genetics, Cell Biology and Development, University of Minnesota, 3Developmental Biology Center, University of Minnesota

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Este protocolo versátil describe el aislamiento de células de la cresta neural premigratoria (NCC) a través de la escisión de pliegues neurales craneales de embriones de pollo. Tras el recubrimiento y la incubación, las NCC migratorias emergen de los explantes del pliegue neural, lo que permite la evaluación de la morfología celular y la migración en un entorno 2D simplificado.

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Biología del desarrollo Número 184
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