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Journal / Biochemistry / 액체와 얼음에서 바이러스 어셈블리의 고해상도 이미징…
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Biochemistry

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JoVE Journal Biochemistry
액체와 얼음에서 바이러스 어셈블리의 고해상도 이미징 향상
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액체와 얼음에서 바이러스 어셈블리의 고해상도 이미징 향상

Article DOI: 10.3791/63856-v • 08:31 min • July 20th, 2022
July 20th, 2022 •
*1,2, *1, *1,3, 1,4, 1,4, 5, 6, 1, 7, 8, 2, 9, 1,5,6
1Department of Biomedical Engineering, Pennsylvania State University, 2Department of Materials Science and Engineering, McMaster University, 3Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 4Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, 5Materials Research Institute, Pennsylvania State University, 6Huck Institutes of the Life Sciences, Pennsylvania State University, 7Applications team, Direct Electron, 8Application Scientist, Protochips, Inc., 9Department of Biology, Wake Forest University
* These authors contributed equally

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여기에서는 투과 전자 현미경을 사용하여 나노 스케일에서 액체-EM 및 Cryo-EM 분석에 적합한 바이러스 어셈블리를 준비하는 프로토콜에 대해 설명합니다.

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생화학 이슈 185 투과 전자 현미경 TEM 액체 -EM Cryo-EM 아데노 관련 바이러스 SARS-CoV-2 로타 바이러스 이중층 입자 DLP 구조 생물학
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