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Kryo-elektrontomografi ekstern datainnsamling og subtomografi gjennomsnitt
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Kryo-elektrontomografi ekstern datainnsamling og subtomografi gjennomsnitt

Article DOI: 10.3791/63923-v • 08:55 min • July 12th, 2022
July 12th, 2022 •
1, 1, *1, *1,2,3
1Electron Bio-Imaging Centre, Diamond Light Source Ltd, Harwell Science & Innovation Campus, 2Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, 3Chinese Academy of Medical Sciences Oxford Institute, University of Oxford
* These authors contributed equally

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Denne protokollen beskriver høyoppløselig kryo-elektrontomografi ekstern datainnsamling ved hjelp av Tomo5 og påfølgende databehandling og subtomografi gjennomsnitt ved hjelp av emClarity. Apoferritin brukes som et eksempel for å illustrere detaljerte trinnvise prosesser for å oppnå en kryo-ET-struktur ved 2,86 Å oppløsning.

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