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Isolering og identifikation af vaskulære endotelceller fra forskellige fedtdepoter til downstream-applikationer
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Isolering og identifikation af vaskulære endotelceller fra forskellige fedtdepoter til downstream-applikationer

Article DOI: 10.3791/63999-v • 09:00 min • June 10th, 2022
June 10th, 2022 •
1, 2, 3, 1
1Department of Kinesiology and Applied Physiology, College of Health Sciences, University of Delaware, 2Division of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, 3University of Delaware Flow Cytometry Core, Delaware Biotechnology Institute, University of Delaware

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Denne protokol beskriver en metode til dissektion af musefedtdepoter og isolering og fordøjelse af respektive arterier for at frigøre og derefter identificere endotelcellepopulationen. Frisk isolerede celler, der anvendes i downstream-applikationer, vil fremme forståelsen af vaskulær cellebiologi og mekanismerne for vaskulær dysfunktion.

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Biologi udgave 184 endotelceller fedt vævsfordøjelse flowcytometri
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