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Journal
/
Biology
/
分析黑
腹果蝇
幼虫脂肪体中饥饿诱导的自噬
JoVE Journal
Biology
Author Produced
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JoVE Journal
Biology
Analyzing Starvation-Induced Autophagy in the
Drosophila melanogaster
Larval Fat Body
Please note that all translations are automatically generated.
Click here for the English version.
分析黑
腹果蝇
幼虫脂肪体中饥饿诱导的自噬
DOI:
10.3791/64282-v
•
06:02 min
•
August 04, 2022
•
Kexin Shi
,
Chao Tong
1
Ministry of Education Key Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute
,
Zhejiang University
Chapters
00:04
Introduction
00:40
Drosophila
Crossing and Egg Laying
01:54
Amino Acid Starvation Induces Autophagy
02:51
Third Instar Larvae Dissection and Sample Tissue Processing
04:29
Results: The Patterns of GFP‐Atg8a Puncta in Control,
Mu1,
and
Mu2
Clones
05:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了通过营养消耗
在
黑腹果
蝇幼虫脂肪体中诱导自噬,并使用转基因苍蝇菌株分析自噬的变化。
Tags
Autophagy
Drosophila Melanogaster
Larval Fat Body
Clonal Analysis
Amino Acid Depletion
Third Instar Larvae
20% Sucrose Solution
Dissection
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