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Analyse af sult-induceret autofagi i Drosophila melanogaster Larve Fat Body
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JoVE Journal Biology
Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

Analyse af sult-induceret autofagi i Drosophila melanogaster Larve Fat Body

DOI:
10.3791/64282-v

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06:02 min

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August 04, 2022

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,
1Ministry of Education Key Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute,Zhejiang University

Chapters

  • 00:04Introduction
  • 00:40Drosophila Crossing and Egg Laying
  • 01:54Amino Acid Starvation Induces Autophagy
  • 02:51Third Instar Larvae Dissection and Sample Tissue Processing
  • 04:29Results: The Patterns of GFP‐Atg8a Puncta in Control, Mu1, and Mu2 Clones
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver induktion af autofagi i Drosophila melanogaster larvefedtlegemet via næringsstofudtømning og analyserer ændringer i autofagi ved hjælp af transgene fluestammer.

Tags

AutophagyDrosophila MelanogasterLarval Fat BodyClonal AnalysisAmino Acid DepletionThird Instar Larvae20% Sucrose SolutionDissection

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