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Developmental Biology

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JoVE Journal Developmental Biology
Effiziente Genom-Editierung von Mäusen durch CRISPR-Elektroporation von Zygoten
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Effiziente Genom-Editierung von Mäusen durch CRISPR-Elektroporation von Zygoten

Article DOI: 10.3791/64302-v • 07:17 min • December 16th, 2022
December 16th, 2022 •
1, 1
1Department of Biomedical Sciences, School of Veterinary Medicine, The University of Pennsylvania

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Hier beschreiben wir eine einfache Technik zur effizienten Erzeugung von genetisch veränderten Mäusen namens CRISPR RNP Electroporation of Zygotes (CRISPR-EZ). Diese Methode liefert Editierreagenzien durch Elektroporation in Embryonen mit einer Effizienz von nahezu 100%. Dieses Protokoll ist wirksam bei Punktmutationen, kleinen genomischen Insertionen und Deletionen in Säugetierembryonen.

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Developmental Biology Heft 190
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