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JoVE Journal Developmental Biology
Edición eficiente del genoma de ratones mediante electroporación CRISPR de cigotos
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Edición eficiente del genoma de ratones mediante electroporación CRISPR de cigotos

Article DOI: 10.3791/64302-v • 07:17 min • December 16th, 2022
December 16th, 2022 •
1, 1
1Department of Biomedical Sciences, School of Veterinary Medicine, The University of Pennsylvania

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Aquí, describimos una técnica simple destinada a la generación eficiente de ratones genéticamente modificados llamada CRISPR RNP Electroporación de Cigotos (CRISPR-EZ). Este método proporciona reactivos de edición por electroporación en embriones con una eficiencia cercana al 100%. Este protocolo es efectivo para mutaciones puntuales, pequeñas inserciones genómicas y deleciones en embriones de mamíferos.

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Biología del desarrollo Número 190
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