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Measurement of Heart Contractility in Isolated Adult Human Primary Cardiomyocytes
JoVE Journal
Medicine
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JoVE Journal Medicine
Measurement of Heart Contractility in Isolated Adult Human Primary Cardiomyocytes
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Measurement of Heart Contractility in Isolated Adult Human Primary Cardiomyocytes

DOI:
10.3791/64394-v

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09:17 min

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August 09, 2022

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, , , , , , ,
1AnaBios Corporation

Chapters

  • 00:05Introduction
  • 00:51Laminin Coating Preparation (One Day Before Measuring Contractility)
  • 01:28Preparation of Test Compounds (On the Day of Measuring Contractility)
  • 02:42Plating of Cardiomyocytes (On the Day of Measuring Contractility)
  • 03:30Recording of Cardiomyocyte Contractility
  • 05:13Analysis of Contractility Data
  • 07:44Results: Validating Human Cardiomyocyte Contractility Measurement
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to measure contractility in adult human primary cardiomyocytes from donor hearts with the MyoBLAZER system, a reliable platform for assessing drug-induced changes in contractility during preclinical development.

Tags

Keywords: Heart ContractilityAdult Human Primary CardiomyocytesOptical RecordingDrug EffectsHeart FailureDrug-induced Contractility RisksLamininDMSOGravity Flow Perfusion SystemMicroscope

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