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JoVE Journal Neuroscience
Detectie van abnormaal prioneiwit door immunohistochemie
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Detectie van abnormaal prioneiwit door immunohistochemie

Article DOI: 10.3791/64560-v • 06:38 min • May 5th, 2023
May 5th, 2023 •
1,2, 1, 2, 2, 2, 3, 2, 1, 1, 1, 1, 1
1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS, University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão

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Immunolabeling van abnormaal prioneiwit met behulp van immunohistochemieprotocollen vereist specifieke monster- en anti-PrP-antilichaamvoorbereidingsmethoden. Het huidige protocol beschrijft de belangrijkste stappen in epitoopontmaskering om te zorgen voor een goede PrP-immunolabeling en om niet-specifieke achtergrondkleuring te minimaliseren. Ook houdt deze benadering rekening met bioveiligheidsmaatregelen bij het uitvoeren van immunohistochemische studies met de prion-geïnfecteerde weefsels.

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Neurowetenschappen Nummer 195
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