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Echtzeitanalyse der Bioenergetik in primären humanen retinalen Pigmentepithelzellen mittels hochauflösender Respirometrie
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Echtzeitanalyse der Bioenergetik in primären humanen retinalen Pigmentepithelzellen mittels hochauflösender Respirometrie

Article DOI: 10.3791/64572-v • 09:16 min • February 3rd, 2023
February 3rd, 2023 •
*1,2, *1, 1, 1,2, 1,2, 1,2
1Schepens Eye Research Institute of Mass. Eye and Ear, 2Department of Ophthalmology, Harvard Medical School
* These authors contributed equally

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Der Stoffwechselstatus menschlicher retinaler Pigmentepithelzellen (H-RPE) spiegelt deren Gesundheit und Funktion wider. Hier wird ein optimiertes Protokoll zur Untersuchung des Echtzeit-Stoffwechselflusses von H-RPE mittels hochauflösender Respirometrie vorgestellt.

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Biologie Heft 192 Stoffwechsel Mitochondrien retinales Pigmentepithel Glykolyse oxidative Phosphorylierung Atmung extrazelluläre Ansäuerungsrate Sauerstoffverbrauchsrate hochauflösende Respirometrie
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