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Journal / Immunology and Infection / Virusförökning och cellbaserad kolorimetrisk…
JoVE Journal
Immunology and Infection
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Virusförökning och cellbaserad kolorimetrisk kvantifiering

Article DOI: 10.3791/64578-v • 07:26 min • April 7th, 2023
April 7th, 2023 •
1, 1, 2, 1, 1
1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE), Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya

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Detta protokoll beskriver spridningen av zikavirus (ZIKV) i njurceller från afrikanska gröna apor och kvantifieringen av ZIKV med hjälp av cellbaserade kolorimetriska immundetektionsmetoder i format med 24 brunnar och 96 brunnar (hög genomströmning).

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Immunologi och infektion utgåva 194 Zikavirus (ZIKV) Flavivirus medfödda hjärnabnormiteter Guillain-Barrãs© syndrom virologi fokusbildande analys (FFA) viralt antigen peroxidasimmunfärgningsteknik 24-brunnsformat 96-brunnsformat Foci-storleksoptimering Vero-celler inkubation färgningsprocesser cellfixering permeabilisering blockering antikroppsbindning peroxidassubstrat
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