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Undersøgelse af interaktioner mellem histonmodificerende enzymer og transkriptionsfaktorer in vivo ved fluorescensresonansenergioverførsel
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Undersøgelse af interaktioner mellem histonmodificerende enzymer og transkriptionsfaktorer in vivo ved fluorescensresonansenergioverførsel

Article DOI: 10.3791/64656-v • 11:33 min • October 14th, 2022
October 14th, 2022 •
1, 1, 1
1Department of Biochemistry and Cell Biology, State University of New York

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Fluorescensresonans energioverførsel (FRET) er en billeddannelsesteknik til påvisning af proteininteraktioner i levende celler. Her præsenteres en FRET-protokol for at studere sammenhængen mellem histonmodificerende enzymer med transkriptionsfaktorer, der rekrutterer dem til målpromotorerne til epigenetisk regulering af genekspression i plantevæv.

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Biologi udgave 188 histonmodificerende enzymer (HME'er) transkriptionsfaktorer (TF'er) protein-proteininteraktioner FRET
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