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Imaging Protein-protein Interactions in vivo
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Biology
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Imaging Protein-protein Interactions in vivo

Imaging Protein-protein Interactions in vivo

DOI:
10.3791/2149-v

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11:15 min

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October 10, 2010

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1Biochemistry and Molecular Biology,Virginia Commonwealth University

Chapters

  • 00:00Title
  • 01:10Introduction
  • 01:35Construction of CFP and YFP Chimeric Receptors and Transfection of EA.hy 926 Cells
  • 04:44Confocal Imaging and Live FRET Analysis
  • 08:41Representative Results
  • 10:08Conclusion

Summary

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This protocol describes how to image protein-protein interactions using a FRET-based proximity assay.

Tags

Protein-protein InteractionsIn VivoImagingGenetic Encodable Fluorescent ProteinsGFPRFPFRETProximity AssayReceptor-receptor InteractionsEndothelial Cell Surface

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