Journal
/
Biology
/
Undersøgelse af interaktioner mellem histonmodificerende enzymer og transkriptionsfaktorer in vivo ved fluorescensresonansenergioverførsel
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Undersøgelse af interaktioner mellem histonmodificerende enzymer og transkriptionsfaktorer in vivo ved fluorescensresonansenergioverførsel

DOI:
10.3791/64656-v

•

11:33 min

•

October 14, 2022

•

, ,
1Department of Biochemistry and Cell Biology,State University of New York

Chapters

  • 00:05Introduction
  • 01:02Agroinfiltration
  • 03:21Confocal Microscopy
  • 08:11Results: Specific Interaction of LSH10 with OTLD1 in Nicotiana benthamiana Leaves Detected by SE-FRET and AB-FRET
  • 10:41Conclusion

Summary

Automatic Translation

Automatic Translation

Fluorescensresonans energioverførsel (FRET) er en billeddannelsesteknik til påvisning af proteininteraktioner i levende celler. Her præsenteres en FRET-protokol for at studere sammenhængen mellem histonmodificerende enzymer med transkriptionsfaktorer, der rekrutterer dem til målpromotorerne til epigenetisk regulering af genekspression i plantevæv.

Tags

FRETProtein-protein InteractionAgroinfiltrationNicotiana BenthamianaHistone Modifying EnzymesTranscription FactorsLiving CellFluorescence Resonance Energy Transfer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties

Imaging Protein-protein Interactions in vivo

Real-time overvågning af ligand-receptor interaktioner med Fluorescence Resonance Energy Transfer

In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors

Hurtig Syntese og Screening af kemisk aktiveret transkriptionsfaktorer med GFP-baserede Journalister

Protein-protein interaktioner visualiseret ved bimolekylær Fluorescens komplementering i tobaksprotoplaster og blade

Undersøgelse af protein-protein interaktioner i levende celler Brug Bioluminescens Resonance Energy Transfer

In Vivo 4-Dimensional sporing af hæmatopoietisk stamcelletransplantation og stamcelle i Voksen Mouse calvarie knoglemarv

Read Article