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多孔设备固体培养基上分离秀 丽隐杆线虫 的长期培养和监测
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Long-Term Culture and Monitoring of Isolated Caenorhabditis elegans on Solid Media in Multi-Well Devices
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多孔设备固体培养基上分离秀 丽隐杆线虫 的长期培养和监测

DOI:
10.3791/64681-v

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09:32 min

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December 09, 2022

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, , , , , , ,
1Department of Molecular & Cellular Biology,University of Arizona, 2Department of Bioengineering, School of Engineering and Applied Sciences,University of Pennsylvania

Chapters

  • 00:05Introduction
  • 01:03Preparation of the Multi‐Well Device
  • 03:00Preparation of the Multi‐Well Device for Media Loading
  • 03:29Filling the Wells with Low Melting Nematode Growth Media (lmNGM)
  • 05:53Adding Copper Sulfate to the Moat and Adding Autoclaved Water Crystals
  • 06:55Spotting the Wells with Concentrated Bacteria
  • 07:29Adding Worms to the Multi‐Well Device and Finishing the Preparation of the Device for Long‐Term Use
  • 08:00Results: Correlation of Measured Phenotypes Across Populations in Individual Animals
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

这里介绍的是一种优化的方案，用于在微加工多孔设备中的固体培养基上培养分离的单个线虫。这种方法允许在个体动物的一生中监测与衰老和健康相关的各种表型，包括活动、体型和形状、运动几何形状和存活率。

Tags

Keywords: Caenorhabditis ElegansLong-term CultureMulti-well DevicesAgingMolecular ProcessesPhysiological ProcessesPDMSMoldVacuum ChamberCuringSterilizationAutoclaving

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