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Chapters
- 00:05Introduction
- 01:03Preparation of the Multi‐Well Device
- 03:00Preparation of the Multi‐Well Device for Media Loading
- 03:29Filling the Wells with Low Melting Nematode Growth Media (lmNGM)
- 05:53Adding Copper Sulfate to the Moat and Adding Autoclaved Water Crystals
- 06:55Spotting the Wells with Concentrated Bacteria
- 07:29Adding Worms to the Multi‐Well Device and Finishing the Preparation of the Device for Long‐Term Use
- 08:00Results: Correlation of Measured Phenotypes Across Populations in Individual Animals
- 08:55Conclusion
Her præsenteres en optimeret protokol til dyrkning af isolerede individuelle nematoder på faste medier i mikrofabrikerede multibrøndsenheder. Denne tilgang gør det muligt at overvåge individuelle dyr gennem hele deres liv for en række fænotyper relateret til aldring og sundhed, herunder aktivitet, kropsstørrelse og form, bevægelsesgeometri og overlevelse.
