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Chapters
- 00:05Introduction
- 01:03Preparation of the Multi‐Well Device
- 03:00Preparation of the Multi‐Well Device for Media Loading
- 03:29Filling the Wells with Low Melting Nematode Growth Media (lmNGM)
- 05:53Adding Copper Sulfate to the Moat and Adding Autoclaved Water Crystals
- 06:55Spotting the Wells with Concentrated Bacteria
- 07:29Adding Worms to the Multi‐Well Device and Finishing the Preparation of the Device for Long‐Term Use
- 08:00Results: Correlation of Measured Phenotypes Across Populations in Individual Animals
- 08:55Conclusion
Presentert her er en optimalisert protokoll for dyrking av isolerte individuelle nematoder på faste medier i mikrofabrikerte flerbrønnsenheter. Denne tilnærmingen gjør det mulig å overvåke individuelle dyr gjennom hele livet for en rekke fenotyper relatert til aldring og helse, inkludert aktivitet, kroppsstørrelse og form, bevegelsesgeometri og overlevelse.
